Calcium electroporation efficiency dependence on calcium concentration and administration time

Calcium ion distribution between extra and intracellular compartments plays a crucial role in cellular response to various external stress conditions. Necrotic and apoptotic processes are associated with intracellular calcium ion overload, because once calcium ions reach their threshold in the cytoplasm they cause changes in activity of enzymes, proteins and organelles ultimately leading to cell death. Intracellular calcium levels leading to cell death can be regulated by electroporation. This calcium electroporation is currently investigated as a novel anticancer treatment. In this study, we have investigated Chinese hamster ovary (CHO) cell viability dynamics after calcium electroporation. Cells were electroporated using 1 high voltage (1400 V/cm, 100 µs) electric pulse in the presence of CaCl2 at different concentrations (0; 0.25; 0.5; 1 mM) and different administration time points after cell treatment with electric pulses (0; 30; 60; 120; 240; 300; 600 s). Our results have shown that after electroporation cell viability was decreasing with increasing CaCl2 concentration and was dependent on administration time. Further evaluation of cell viability in dependence of cytotoxic agent administration time after electroporation in the presence of calcium ions shows that calcium ions can affect pore resealing dynamicsAplinkos tyrimų centrasBiologijos katedraGamtos mokslų fakultetasVytauto Didžiojo universiteta