U937 cell response to calcium electroporation.

<p>U937, a human leukaemia cell line. (<b>A</b>) Cellular quinacrine fluorescence intensity of U937 15 min after calcium electroporation. The extracellular quinacrine concentration was 10 μM during loading and electroporation. Final extracellular calcium concentrations of 0 mM or 3 mM and applied electric field of 1.0 kV/cm were used. Columns depict the average of 180 cells (9 experiments × 20 cells). Arbitrary units (AU), mean + S.D., n = 9. (<b>B</b>) U937 cell viability measured 24 h after calcium electroporation. Viability assessed for U937 using resazurin cell proliferation assay. Extracellular calcium concentrations of 0 or 3 mM. Applied electric field of 1.0 kV/cm. Results are illustrated as percentage of control (no electroporation, no added calcium), mean + S.D., n = 6.