In this work, a new type of penicillin G acylase (PGA)-based monolithic silica support was developed and evaluated for the chiral separation in HPLC. The preparation procedure consisted of two steps: preparation of an epoxy derivatized monolithic silica column and chemical modification of the epoxide groups with the enzyme chiral selector. The epoxy Silica-Rod column for the immobilization of PGA was prepared with the in situ modification process by using epoxy-silanes and the identification of the species bound to the surface was achieved by solid-state nuclear magnetic resonance. The enzyme was covalently immobilized to the surface of the derivatized monolithic column. The enantioselectivity and the performance of the developed colum...
Penicillin G acylase is the second most important enzyme used by industry in an immobilized form. Pe...
Two newchiral and restricted-access materials containing glycopeptide antibiotics as chiral selector...
The objective of this work was to develop a hydrogel-coated monolith for the entrapment of penicilli...
In this work, a new type of penicillin G acylase (PGA)-based monolithic silica support was developed...
An epoxy derivatized monolithic silica capillary column (100 micron i.d.) was used as a support for ...
An epoxy derivatized monolithic silica capillary column (100 micron i.d.) was used as a support for ...
An epoxy derivatized monolithic silica capillary column (100 micron i.d.) was used as a support for ...
This review draws attention to the use of chiral monolithic silica HPLC columns for the enantiomeric...
A technique based on liquid chromatography has been developed to facilitate studies of enantioselect...
A technique based on liquid chromatography has been developed to facilitate studies of enantioselect...
A technique based on liquid chromatography has been developed to facilitate studies of enantioselect...
A technique based on liquid chromatography has been developed to facilitate studies of enantioselect...
Enantiomeric separations in CEC with the macrocyclic antibiotic vancomycin immobilized silica monoli...
Chondroitin sulfate A was covalently immobilized onto a monolithic silica epoxy column involving a S...
Alcaligenes faecalis penicillin G acylase has several desired features over other penicillin G acyla...
Penicillin G acylase is the second most important enzyme used by industry in an immobilized form. Pe...
Two newchiral and restricted-access materials containing glycopeptide antibiotics as chiral selector...
The objective of this work was to develop a hydrogel-coated monolith for the entrapment of penicilli...
In this work, a new type of penicillin G acylase (PGA)-based monolithic silica support was developed...
An epoxy derivatized monolithic silica capillary column (100 micron i.d.) was used as a support for ...
An epoxy derivatized monolithic silica capillary column (100 micron i.d.) was used as a support for ...
An epoxy derivatized monolithic silica capillary column (100 micron i.d.) was used as a support for ...
This review draws attention to the use of chiral monolithic silica HPLC columns for the enantiomeric...
A technique based on liquid chromatography has been developed to facilitate studies of enantioselect...
A technique based on liquid chromatography has been developed to facilitate studies of enantioselect...
A technique based on liquid chromatography has been developed to facilitate studies of enantioselect...
A technique based on liquid chromatography has been developed to facilitate studies of enantioselect...
Enantiomeric separations in CEC with the macrocyclic antibiotic vancomycin immobilized silica monoli...
Chondroitin sulfate A was covalently immobilized onto a monolithic silica epoxy column involving a S...
Alcaligenes faecalis penicillin G acylase has several desired features over other penicillin G acyla...
Penicillin G acylase is the second most important enzyme used by industry in an immobilized form. Pe...
Two newchiral and restricted-access materials containing glycopeptide antibiotics as chiral selector...
The objective of this work was to develop a hydrogel-coated monolith for the entrapment of penicilli...