POTENSI MINYAK ESENSIAL KULIT BUAH CITRUS LIMON L. 1% TERHADAP PENINGKATAN FGF-2 DAN FIBROBLAS PADA ULSER MUKOSA MULUT TIKUS DENGAN STRES OKSIDATIF

Background: High oxidative stress can interfere the healing process of oral mucosal ulcers, therefore required a therapeutic substance that can act as an antioxidant. Essential oil of Citrus Limon L. fruit peel has antioxidant potential through a high content of limonene. Until now there has been no research on the use of essential oil of Citrus Limon L. fruit peel as a medicinal herb in oral mucosal ulcers. Study Objectives: Proving the potential of 1% essential oil of Citrus Limon L. fruit peel topically to the oral mucosa ulcer healing process through increased expression of FGF-2 and the number of fibroblasts in Wistar rats (Rattus norvegicus) with oxidative stress. Methods: Thirty Wistar rats exposured to cigarette smoke for 30 days, oral ulcer made on rats mucosa and divided into three treatment groups were treated with 1% essential oil of Citrus Limon L. fruit peel and three control groups without treatment. Decapitation was carried out on day 5th, 7th and 9th and then made preparations for histopathological examination to count FGF-2 expression and the number of fibroblasts. Result: Independent T-test showed a significant difference between the expression of FGF-2 treatment groups compared to controls at day 5th, 7th and 9th. Differences in the number of fibroblasts significantly on day 7 and 9. Conclusion: Application of 1% essential oil of Citrus Limon L. fruit peel on the labial mucosa ulceration of wistar rats that experience oxidative stress can increase the expression of fibroblast growth factor- 2 (FGF-2) and the number of fibroblasts. Keywords: essential oil, Citrus limon L. antioxidant, oral ulcer