Spiders were collected from various habitats in 7 counties of northwestern Iowa during the summer of 1985. Nineteen families and 69 genera were identified; these comprised 154 species. An additional 19 species in Families Linyphiidae and Micryphantidae were not identified to genus. Morphological keys to local families and genera are presented. A field key to some common spiders, based on ecological and behavioral characteristics, is also included