This study uses groundwater isotopes and ion composition to verify model simulations and ecohydrological studies in the Drentsche Aa nature reserve in The Netherlands, which is representative for the northwestern wetland areas in the Ice Marginal Landscape zone. At eight field sites, a total of 24 samples were analysed for their 13 C, 14 C, 2 H, and 18 O isotopes and ionic composition. The isotopes indicate that most of the fen peatlands in the area depend on the exfiltration of sub-regional groundwater flows, which confirmed the previous model simulations and ecohydrological studies. At three sites, isotopes and ionic composition indicate that the groundwater from the sub-regional system has been replaced by local infiltrated rainwater, du...