Confocal microscopy.
- Amanda Whitmill (5680232)
- Seongcheol Kim (198951)
- Vivian Rojas (5680235)
- Fahad Gulraiz (586860)
- Kazi Afreen (5680238)
- Mamta Jain (623611)
- Meharvan Singh (5680241)
- In-Woo Park (5656204)
Publication date
August 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
Subcellular localization was determined by confocal microscopic analysis after transfecting the indicated genes together with pDsRed2-ER (Clontech) into Huh7.5.1 and its replicon cells, APC140. Expression of each protein was detected by anti-Myc Ab for ACSL4 (A) and IFI27 (C) and by anti-GNMT Ab for GNMT (B) followed by Alexa Fluor 488. Thus, expression of the cellular genes shows green color, and expression of pDsRed2-ER encodes a fusion consisting of red fluorescence protein, showing red color in the endoplasmic reticulum (ER) of the transfected cells; DAPI depicts the nucleus.</p
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