Colony forming ability of cells at passage 2.

(A) Experimental design. Passage-2 cells were plated at 1 × 103 cells/dish (60 cm2 dishes) in 6 dishes. After 14 days of culture, three dishes were stained with crystal violet for colony counting and the other three dishes were used for cell counting and further analyses. (B) Images of colonies stained with crystal violet. (C) Colony number (1 × 105)/dish (60 cm2 dishes). (E) Cell number/colony. The means ± SD are shown (n = 18). *, p < 0.05. **, p < 0.01 with the Kruskal Wallis test, followed by Dunn’s multiple comparisons test.