<p>Cells were plated in culture dishes as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029709#s2" target="_blank">Materials and Methods</a>. Colonies were scored and photographed 20 days after plating. The terms “microcolony” and “large” indicate the dominating size type of all established colonies, which only showed limited size variations.</p
Accurate descriptions of the kinetics of cell growth in semi-solid agar clonogenic systems have been...
1<p>Cells were seeded and the drugs (25 µM) were added 24 h later. After 72 h of treatment, the cell...
<p>Cells were plated at a density of 1×10<sup>5</sup> cells in 6-cm dishes. A–C, Numbers of cells in...
<p>A total of 1.5×10<sup>4</sup> Ishikawa cells and 1.2×10<sup>4</sup> USPC-1 cells were seeded on 6...
<p>(A) 1×10<sup>4</sup> cells were plated into soft agar in 6-well plates. Cells were observed for 1...
<p>(A) Soft agar colony formation assays were performed on each clone as a measure of cell-adhesion ...
<p>(<b>A</b>) A colony in soft agar, scale bar = 10 µm; (<b>B</b>) A colony in soft agar, scale bar ...
<p>Agar colonies were obtained by culture of sorted cells from mouse mammary carcinoma Cl66 cells cu...
<p>(A, B) Equal numbers of parental U251, U251-neo, U251-NC and U251-S cells were seeded onto 60 mm ...
(A) Experimental design. Passage-2 cells were plated at 1 × 103 cells/dish (60 cm2 dishes) in 6 dish...
Celigo Image cytometer counts of colonies developed after 3 days in culture from suspensions of AGMK...
Photographs were taken at 4X magnification using a BZ-9000 BioRevo microscope.</p
<p>The cell suspensions (2×10<sup>4</sup> cells) mixed with 0.3% agarose and different concentration...
<p>(a) A soft agar assay was used to detect the anchorage independent growth of transformed cells fr...
<p>(A) Proliferation rate in culture-treated plates was determined after 96 hours by using the XTT a...
Accurate descriptions of the kinetics of cell growth in semi-solid agar clonogenic systems have been...
1<p>Cells were seeded and the drugs (25 µM) were added 24 h later. After 72 h of treatment, the cell...
<p>Cells were plated at a density of 1×10<sup>5</sup> cells in 6-cm dishes. A–C, Numbers of cells in...
<p>A total of 1.5×10<sup>4</sup> Ishikawa cells and 1.2×10<sup>4</sup> USPC-1 cells were seeded on 6...
<p>(A) 1×10<sup>4</sup> cells were plated into soft agar in 6-well plates. Cells were observed for 1...
<p>(A) Soft agar colony formation assays were performed on each clone as a measure of cell-adhesion ...
<p>(<b>A</b>) A colony in soft agar, scale bar = 10 µm; (<b>B</b>) A colony in soft agar, scale bar ...
<p>Agar colonies were obtained by culture of sorted cells from mouse mammary carcinoma Cl66 cells cu...
<p>(A, B) Equal numbers of parental U251, U251-neo, U251-NC and U251-S cells were seeded onto 60 mm ...
(A) Experimental design. Passage-2 cells were plated at 1 × 103 cells/dish (60 cm2 dishes) in 6 dish...
Celigo Image cytometer counts of colonies developed after 3 days in culture from suspensions of AGMK...
Photographs were taken at 4X magnification using a BZ-9000 BioRevo microscope.</p
<p>The cell suspensions (2×10<sup>4</sup> cells) mixed with 0.3% agarose and different concentration...
<p>(a) A soft agar assay was used to detect the anchorage independent growth of transformed cells fr...
<p>(A) Proliferation rate in culture-treated plates was determined after 96 hours by using the XTT a...
Accurate descriptions of the kinetics of cell growth in semi-solid agar clonogenic systems have been...
1<p>Cells were seeded and the drugs (25 µM) were added 24 h later. After 72 h of treatment, the cell...
<p>Cells were plated at a density of 1×10<sup>5</sup> cells in 6-cm dishes. A–C, Numbers of cells in...