Additional file 6: of Overexpression of a cell wall damage induced transcription factor, OsWRKY42, leads to enhanced callose deposition and tolerance to salt stress but does not enhance tolerance to bacterial infection

Figure S6. Ectopic expression of OsWRKY42 in Arabidopsis alters expression of genes involved in heteroxylan biosynthesis but does not affect expression of different callose synthase genes. Leaves of three weeks old plants were infiltrated either with inducer (20 μM 17-β-estradiol) or water using a 1 ml needleless syringe. Twelve hours post infiltration, leaves were harvested and processed for qPCR analysis. The graph represents relative fold change (2-∆∆Ct) using expression values of Est treated over water treated samples. AtUBQ5 was used as an internal control for qPCR analysis. The error bar represents standard deviation. All of the above experiments were repeated in two independent transgenic lines. (TIF 2938 kb