Additional file 5: of Parkinson’s disease-linked Parkin mutations impair glutamatergic signaling in hippocampal neurons

Figure S5. Parkin has no effect on phospho-Serine 845 GluA1 reduction during cLTD. (a) Representative immunoblots of lysates from 14 DIV hippocampal neurons expressing GFP/shParkin/shParkin-WT/-T240M/-R334C constructs, under control condition (−) or after cLTD induction (+) and probed with the indicated antibodies. (b) Quantification of the ratio of phospho-Serine 845 GluA1 intensity with cLTD to control condition, normalized to GFP control. (n = 3 experiments, one-way ANOVA, error bars represent SEM). (PDF 119 kb