Additional file 1: of Parkinson’s disease-linked Parkin mutations impair glutamatergic signaling in hippocampal neurons

Figure S1. Expression of Parkin mutants in hippocampal neurons. (a) Schematic diagram of Parkin showing domains and mutation/deletion sites. (b) Immunoblots of lysates from 14 DIV hippocampal neurons expressing GFP control, shParkin, shParkin-WT, shParkin-T240M, shParkin-R275W, shParkin-R334C, or shParkin-G430D constructs, probed for Parkin, GFP, and tubulin. (c) Representative images of shParkin-WT, shParkin-T240M, shParkin-R334C, shParkin-R275W and shParkin-G430D expression in 14 DIV hippocampal neurons. Scale bar, 10 μm. (d) Immunoblots of lysates from 14 DIV Parkin KO hippocampal neurons expressing shParkin, shParkin-WT, shParkin-T240M, shParkin-R275W, shParkin-R334C or shParkin-G430D constructs and Parkin KO control, probed for Parkin, GFP, and tubulin. (e) Immunoblots of lysates from hippocampal neurons expressing GFP, shParkin, shParkin-WT, shParkin-C431S, or shParkin-W403A, probed for Parkin, GFP, and tubulin. Note that GFP-Parkin W403A is not recognized by the Parkin antibody but is detected by the GFP antibody. (PDF 3931 kb