PLP dynamics in the binding pocket of hGOT1.

(A) PLP RMSD values based on four 1 μs simulations of WT, E266K, R267H, and P300L. The blue plot represents WT, whereas E266K is shown in yellow, R267H is shown in purple, and P300L is shown in green. (B) RMSD distribution of PLP in each variant and WT. WT shows a much narrower distribution than the 3 variants. The color scheme is the same as in panel A. The representative PLP snapshots in the pocket are shown by (C) WT, (D) E226K, (E) R26H, and (F) P300L. The opposite monomer is colored in purple, and the amino acid substitutions are shown in green.