Maximum likelihood phylogenetic tree of HIV-1 subtype B <i>pol</i> (~1000 pb) sequences belonging to the major Guatemalan clades (B_GU-I to B_GU-X, n = 787), and reference sequences from various Central American countries (<i>n</i> = 694), Panama (<i>n</i> = 583), and the Caribbean (<i>n</i> = 238).

Branches are colored according to the geographic origin of each sequence, as indicated in the legend (top left). Colored boxes highlight the position of the six major Guatemalan HIV-1 subtype B clades (BGUs) and the four Central American clades (BCAM-I to BCAM-VI). The number of sequences and aLRT support values for each clade are indicated at the bottom. The tree was rooted using HIV-1 subtype D reference sequences. Branch lengths are drawn to scale with the bar at the bottom indicating nucleotide substitutions per site.