Additional file 1: of Alternative splicing links histone modifications to stem cell fate decision

Figure S1. Identifying hESC differentiation-related AS exons. Figure S2. The hESC differentiation-related AS exons possess the typical properties of AS exons. Figure S3. AS profiles upon hESC differentiation show lineage-specific splicing pattern. Figure S4. HMs change significantly around the alternatively spliced exons upon hESC differentiation. Figure S5. A subset of AS events are significantly associated with some HMs upon hESC differentiation. Figure S6. K-means clustering based on selected epigenetic features of eight HMs for MXE and SE AS exons. Figure S7. HM-associated AS genes are more lineage-specific. Figure S8. HM-unassociated AS genes are enriched in G1 cell-cycle phase and pathways for self-renewal. Figure S9. Isoform switch from PBX1a and PBX1b during hESC differentiation. Figure S10. Isoform switch of PBX1 links H3K36me3 to hESC fate decision. Figure S11. The effect of ΔPSI cutoffs for AS-HM correlations. Table S1. The number of all AS events identified during hESC differentiation. Table S5. The PCR primers used in this study. (PDF 1917 kb