CD43 deficiency does alter numbers of CD4⁺ or CD8⁺ T cells during sepsis.

B6 and CD43-/- mice were subjected to CLP and spleens were harvested at 24h and analyzed by flow cytometry. A) Representative flow cytometry of frequencies of CD4+ and CD8+ T cells. B) Summary data of numbers of CD4+ T cells revealed a significant decrease in CD4+ T cell counts in septic CD43-/- mice compared to sham CD43-/- mice (33345±5137 vs. 63792±8225, p = 0.007). However, there were no differences between the sham and septic groups in WT mice (51629±2559 vs 39455±6336, p = 0.4), nor between the two septic groups (25918±1500 vs 23377±3698, p>0.9). C) Summary data of numbers of CD8+ T cells revealed no difference in CD8+ T cell counts between CD43-/- sham and WT sham groups (37208±5632 vs 40318±3567, p = 0.95). Similarly, there was no difference in the number of CD8+ T cells between the septic groups (24609±5684 vs 36618±5539, p = 0.4). Data shown are cumulative from 2 separate experiments with a total of 9-11animals per group. Statistical comparisons were performed by one-way ANOVA followed by Kruskall-Wallis non-parametric post-tests.