Cytotoxicity of free DOX and DOX from ThermoDox at different temperatures with and without RT.

Surviving fraction of HT1080 cells exposed to DOX (0.02 μg/mL) or ThermoDox (0.02 μg/mL) for 1 hour at 37°C or 43°C without (A) or with (B) RT. ThermoDox at 37°C, with or without RT, resulted in equal toxicity compared to treatment without a drug, however ThermoDox at 43°C resulted in comparable toxicity compared to DOX treatment.