Cytotoxicity of hyperthermia at different temperatures and DOX.

(A) Surviving fraction as function of DOX concentration for 1 hour at 37, 42 or 43°C. Cells were exposed for 1 hour to DOX at 37, 42 and 43°C. Both 42 and 43°C showed enhancement cytotoxicity of the DOX, which was the largest at 43°C. (B) Percentage γH2AX positive cells 2 and 26 hours after incubation with DOX (0, 0.02 or 0.06 μg/ml) for 1 hour at 37°C or 43°C. HT enhanced the percentage γH2AX positive cells both 2 and 26 hours after treatment.