(A) Vibrio ZWU0020 pomAB merodiploids robustly express GFP. After outgrowth and plating on nonselective media, mosaic merodiploid colonies exhibiting loss of GFP and thus, loss of the allelic exchange vector, can be isolated. Images labeled “GFP” show GFP fluorescence and “darkfield” show total colony structure. (B) Plotted is the average optical density at 600nm (OD600) vs. time (hours) of WT ZWU0020 and its ΔpomAB derivative during shaking growth in LB broth at 30°C. Range bars are based on four technical replicates.</p
<p>Harveyi clade <i>Vibrio</i> strain BB120 (A) and derivative mutants JMH603 (B), KM387 (C) and JAF...
<p>(A) Colony morphology of the wild type (PH-1), <i>FgATG9</i> deletion mutants (Δ<i>Fgatg9-2</i>, ...
WT Vibrio carrying pMMb67 plasmid without GFP-MreB<strong>Tilt Series Date:</strong> 201...
Five replicate cultures of GFP-positive Vibrio ZWU0020 pomAB merodiploids were grown overnight in LB...
Five replicate cultures of GFP-positive Vibrio ZOR0035 cheA merodiploids were grown overnight in LB ...
Vibrio ZWU0020 (top row) and Aeromonas ZOR0001 (bottom row) merodiploid cells were plated on non-sel...
(A) Top: wild-type pomAB locus in Aeromonas veronii ZOR0001. Bottom: result of markerless pomAB dele...
Five replicate cultures of GFP-positive Aeromonas ZOR0001 pomAB merodiploids were grown overnight in...
(A) Relative luxCDABE output over time from the 882 luxO882 (cyan), 882 luxO+ (orange), 882 luxO882-...
A flagellum‐negative mutant, M8.2, of the marine bacterium Vibrio sp. S141 was produced by transposo...
Aim: Our goal was to develop a simple system for tagging wild-type marine bacteria with gfp. Methods...
Additional file 4: Figure S1. Yield efficiency of transformation for vcIpk, vcIIpk and vcIIIpk const...
Green fluorescent protein (GFP) has been used extensively for in situ animal studies that follow up ...
The symbiosis between the gram-negative marine bacterium Vibrio fischeri and its host Euprymna Scolo...
(A) Pie chart illustrating stratification of S. pombe gene deletion collection into non-essential an...
<p>Harveyi clade <i>Vibrio</i> strain BB120 (A) and derivative mutants JMH603 (B), KM387 (C) and JAF...
<p>(A) Colony morphology of the wild type (PH-1), <i>FgATG9</i> deletion mutants (Δ<i>Fgatg9-2</i>, ...
WT Vibrio carrying pMMb67 plasmid without GFP-MreB<strong>Tilt Series Date:</strong> 201...
Five replicate cultures of GFP-positive Vibrio ZWU0020 pomAB merodiploids were grown overnight in LB...
Five replicate cultures of GFP-positive Vibrio ZOR0035 cheA merodiploids were grown overnight in LB ...
Vibrio ZWU0020 (top row) and Aeromonas ZOR0001 (bottom row) merodiploid cells were plated on non-sel...
(A) Top: wild-type pomAB locus in Aeromonas veronii ZOR0001. Bottom: result of markerless pomAB dele...
Five replicate cultures of GFP-positive Aeromonas ZOR0001 pomAB merodiploids were grown overnight in...
(A) Relative luxCDABE output over time from the 882 luxO882 (cyan), 882 luxO+ (orange), 882 luxO882-...
A flagellum‐negative mutant, M8.2, of the marine bacterium Vibrio sp. S141 was produced by transposo...
Aim: Our goal was to develop a simple system for tagging wild-type marine bacteria with gfp. Methods...
Additional file 4: Figure S1. Yield efficiency of transformation for vcIpk, vcIIpk and vcIIIpk const...
Green fluorescent protein (GFP) has been used extensively for in situ animal studies that follow up ...
The symbiosis between the gram-negative marine bacterium Vibrio fischeri and its host Euprymna Scolo...
(A) Pie chart illustrating stratification of S. pombe gene deletion collection into non-essential an...
<p>Harveyi clade <i>Vibrio</i> strain BB120 (A) and derivative mutants JMH603 (B), KM387 (C) and JAF...
<p>(A) Colony morphology of the wild type (PH-1), <i>FgATG9</i> deletion mutants (Δ<i>Fgatg9-2</i>, ...
WT Vibrio carrying pMMb67 plasmid without GFP-MreB<strong>Tilt Series Date:</strong> 201...