FIG S10. Quantification of second recombination events in Vibrio ZWU0020 at the pomAB locus.

Five replicate cultures of GFP-positive Vibrio ZWU0020 pomAB merodiploids were grown overnight in LB without antibiotic selection. Serial dilutions of each culture were plated on TSA at a density that allowed discrete colonies to form. The frequency at which colonies contained detectable loss of GFP expression was determined by visually inspecting plates using a fluorescence stereomicroscope. Plotted is the distribution of frequencies across all replicate cultures of colonies with complete GFP loss or partial GFP loss. Bars represent median frequency and interquartile range. The raw values used to generate this plot are reported in Data S1.