CGRP release from capsaicin stimulated neurites under different growth factor conditions.

(A) Time course of CGRP release in response to capsaicin stimulation (300 nM) for 5 min (grey shading). Neurites were cultured with NGF (● n = 13/2 compartments/animals) or GDNF (■ n = 11/2). Somata were cultured with NGF in both cases. Inset: CGRP release as AUC (pg/ml) determined from the respective time course experiments. (B) Comparison between Capsaicin (300 nM, grey columns) and potassium (60mM, black columns) stimulated neurites, grown under NGF or GDNF, respectively. Somata were cultured with NGF in both cases. Data were taken from Figs 5B and 6A. Error bars indicate ± SEM. Asterisks indicate p<0.05.