(A) Values for the individual compartments following neurite stimulation (filled symbols) and soma stimulation (open symbols). Symbols indicate the added growth factors in the chambers; N-N-N denotes NGF in the lateral and central compartments (circles); G-N-G denotes GDNF in the lateral compartments and NGF in the central compartment (squares); Ø-G-Ø denotes no growth factor in the lateral compartments and GDNF in the central compartment (triangles). Data derived from the experiments presented in Fig 4. (B) Mean values from data presented in A. Error bars indicate ± SEM; asterisks indicate p<0.05.</p
(A) U251 cells treated with 50 ng/ml GDNF for 0.5, 24 and 48 h and the control group were stained an...
<p>All data represent mean ± SD. *<i>p</i><0.05 between control and others (NGF/CCH and NGF–CMSs/CCH...
<p>(<b>A, B</b>) Double labeling for ATF3 (green) and GFAP (red), in L5 DRGs sections from a control...
(A) Time course of CGRP release in response to capsaicin stimulation (300 nM) for 5 min (grey shadin...
CGRP release from potassium-stimulated neurites (A) or somata (C) and release in the adjacent soma (...
(A) Number of lanes with outgrown neurites per lateral compartment. (B) Total length of the neurites...
<p>All neurotrophic factor treatments caused significantly longer neurites compared to the neurons o...
Basal CGRP secretion; n: Number of compartments/animals; values represented as pg/ml (mean ± SEM).</...
<p>(A) Bar graph showing mean GnRH neurite lengths at 1 and 2 DIV across the three different treatme...
Significant responses are indicated with asterisks (one way ANOVA followed by Dunnett's test; * – < ...
<p>DRG explants and dissociated cells grown in collagen for 48 h with various NGF concentrations wer...
Additional file 2: Fig.S1. Characterization of Ctrl, Sema 3A-GFP, siSema 3A, siNpn 1 and Sema 3A+siN...
<p>The number of neurites observed on control are compared to those seen with three different levels...
<p>A) Immunostaining of β-tubulin III (red) in immature neurons differentiated form NPCs. Scale bars...
<p>The mRNA levels of NF-200 and GAP-43 increased in neuromuscular coculture as compared with that i...
(A) U251 cells treated with 50 ng/ml GDNF for 0.5, 24 and 48 h and the control group were stained an...
<p>All data represent mean ± SD. *<i>p</i><0.05 between control and others (NGF/CCH and NGF–CMSs/CCH...
<p>(<b>A, B</b>) Double labeling for ATF3 (green) and GFAP (red), in L5 DRGs sections from a control...
(A) Time course of CGRP release in response to capsaicin stimulation (300 nM) for 5 min (grey shadin...
CGRP release from potassium-stimulated neurites (A) or somata (C) and release in the adjacent soma (...
(A) Number of lanes with outgrown neurites per lateral compartment. (B) Total length of the neurites...
<p>All neurotrophic factor treatments caused significantly longer neurites compared to the neurons o...
Basal CGRP secretion; n: Number of compartments/animals; values represented as pg/ml (mean ± SEM).</...
<p>(A) Bar graph showing mean GnRH neurite lengths at 1 and 2 DIV across the three different treatme...
Significant responses are indicated with asterisks (one way ANOVA followed by Dunnett's test; * – < ...
<p>DRG explants and dissociated cells grown in collagen for 48 h with various NGF concentrations wer...
Additional file 2: Fig.S1. Characterization of Ctrl, Sema 3A-GFP, siSema 3A, siNpn 1 and Sema 3A+siN...
<p>The number of neurites observed on control are compared to those seen with three different levels...
<p>A) Immunostaining of β-tubulin III (red) in immature neurons differentiated form NPCs. Scale bars...
<p>The mRNA levels of NF-200 and GAP-43 increased in neuromuscular coculture as compared with that i...
(A) U251 cells treated with 50 ng/ml GDNF for 0.5, 24 and 48 h and the control group were stained an...
<p>All data represent mean ± SD. *<i>p</i><0.05 between control and others (NGF/CCH and NGF–CMSs/CCH...
<p>(<b>A, B</b>) Double labeling for ATF3 (green) and GFAP (red), in L5 DRGs sections from a control...