<i>FoxO6</i> expression during mouse embryonic development.

A) Schematic diagram of FoxO6 knockout strategy. The targeting construct carries a LacZ reporter gene and a constitutively expressed neomycin resistance gene (Neo) embedded in two loxp sites. The entire FoxO6 coding region from start codon to stop codon is replaced by LacZ-Neo (ES cells obtained from KOMP biorepository). B-F) FoxO6 expression is shown by X-gal staining in FoxO6+/- mice. B) FoxO6 expression is not detectable at E10.5. C) At E12.5, FoxO6 expression is observed in the brain (frontal lobes, FL; cerebellum primordium, CP; trigeminal ganglion, TG; somites, S. D) At E14.5, FoxO6 expression is in the brain, craniofacial regions, also in the posterior maxilla (PMx) and posterior mandible (PMn). E) At E18.5, FoxO6 has strong expression in the craniofacial region, such as mandibular bone(MB), incisor (IN), molar (M) and palate (PL).