Additional file 7: of Acidic microenvironment plays a key role in human melanoma progression through a sustained exosome mediated transfer of clinically relevant metastatic molecules

Figure S6. MNI and MI intracellular expression of exosome markers and intracellular pH (pHi) evaluation a-c. MNI, and d-f MI cells were left untreated or pH 6.0 treated for the indicated times, then cells were lysed and 30 μg/lane analyzed for Alix and Tsg101 by western blot. a, d representative western blots are showed. b, e Alix, and c, f Tsg101 expression were normalized against tubulin by densitometry analysis and expressed as fold increase (n = 3),. g pHi measurement. Cells were left untreated or incubated at pH 6.0. After 24 h intracellular pH was measured with 3 μM BCECF-AM for 45 min at 37 °C. Mean ± s.d. (n = 3) *, p < 0,05 **, p < 0,01. (PNG 485 kb