Immunogenicity screen of GP vaccines in mice.

C57BL/6 mice (n = 5) were immunised with each of the GP vaccine combinations shown on the x-axis of panel A. The GP “cocktail” vaccine was comprised of a combination of FTT0071, FTT0438, FTT0814 and IglC. Panel A: IgG1 and IgG2c antibody isotypes recognising antigens in a LVS lysate were measured by ELISA in individual serum samples collected 2 weeks after the second vaccination. The mean IgG1 and IgG2c responses (ng/ml) are presented as a stacked bar graph showing the mean response for each vaccine group (+ SEM). Panel B: Antigen stimulated expression of IFNγ was measured by ELISPOT. Spleens were harvested from mice 6 weeks after the final vaccination and splenocytes from individual mice cultured with the antigen corresponding to the immunising GP candidate. Expression of IFNγ was measured by ELISPOT and presented for both medium (white bars / circles) and antigen (black bars/circles) stimulated splenocytes. The circles are the responses for individual mice and the bars are the mean response for the group. Where the antigen-specific response is significantly elevated relative to the medium control, this is indicated (*** p<0.001, t-test with Holm-Sidak multiple comparison correction).