Repression of TXNIP, NF-κB and oxidative stress in cGVHD-susceptible organs by oral administration of TL.

(a) Immunoblot investigation into TXNIP, NF-κB and 4-HNE was conducted. (BM+SC Lanes 1, 3, 5, 7: TL-treated organs, Lanes 2, 4, 6, 8: vehicle-treated organs, BM only Lanes 9, 11, 13, 15: TL-treated organs, Lanes 10, 12, 14, 16: vehicle-treated organs) Note: A series of immunoblots is shown in S10 Fig, and blots of TXNIP, NF-κB, 4-HNE and β-actin are taken from S10 Fig. (b) TXNIP, NF-κB and 4-HNE in the individual organs were subsequently quantified by densitometry. TL-medicated organs (BM+SC+TL: blue, BM+TL: green) and their vehicle-medicated equivalents (BM+SC+Vehicle: red, BM+Vehicle: black). Data from one of two similar experiments are shown. Unpaired Student’s t-test was used to determine the statistical significance between the 2 groups of interest. One-way ANOVA was utilized for the 4 groups of interest. The data are presented as means, ± SD. BM+SC+TL: n = 10, BM+SC+Vehicle: n = 10, BM+TL: n = 6, BM+Vehicle: n = 6. *P<0.05.