Trastuzumab induces oxidative stress and increases Caspase 3/7 activity in cardiomyocytes.

<p>(<b>a</b>) Quantification of ELISA measurement of NT in the hearts of animals treated with trastuzumab compared to the control vehicle injected animals. (<b>b</b>) Quantification of ELISA measurement of 4-HNE adducts in the hearts of animals treated with trastuzumab compared to the control vehicle injected animals. (<b>c</b>) Quantification of ELISA measurement of caspase 3 and 7 in the trastuzumab-treated animals compared to the control vehicle injected animals. For all the graphs in Figure 4, the results are expressed as fold changes relative to that of control vehicle-treated animals. Student’s t-test was used to compare the two groups and significance is determined as *P < 0.05 or **P< 0.01 vs. the vehicle treated animals. At least six animal were used for each group (n=6).