Analysis of cardiac signaling pathways.

Gene expression time course analysis of tgfb1 (A) and smad2 (B) log2 fold change in expression in IR/saline vs IR/LepA treated mice in the heart. IHC analysis of phosphorylated Smad2 in heart samples from POD-30 collected from IR/saline (C1) and IR/LepA (C2) treated mice. Scale bar 50μm; p2 fold change in expression in IR/saline and IR/LepA POD-30 cardiac samples (F). IHC analysis of phosphorylated STAT3 in heart samples from POD-30 collected from IR/saline (E1) and IR/LepA (E2) treated mice. Scale bar 50μm. Arrowheads point at positive nuclear signal, stained light brown. (5 samples from each group were used for IHC analysis).