Western blot analysis of myocardial IRF5 immunoprecipitates.

<p>Western blot analysis of IRF5 immunoprecipitates from whole heart lysates shows that phosphoserine levels on IRF5 were essentially the same in Tsk^−/+ hearts as the levels on IRF5 in C57BL/6J hearts. 4F treatments reduced phosphoserine levels on IRF5 in Tsk^−/+ hearts compared with the levels on IRF5 in hearts from Tsk^−/+ and C57BL/6J mice. Total ubiquitin on IRF5 was upregulated in Tsk^−/+ hearts and 4F treatments of Tsk^−/+ mice, significantly increased total ubiquitin levels on IRF5 in the Tsk^−/+ hearts compared with the levels on IRF5 in C57BL/6J hearts (* = p<0.05, n = 4). Finally, K⁶³ ubiquitin on IRF5 in Tsk^−/+ hearts is decreased compared to the levels on IRF5 in C57Bl/6J myocardium. 4F treatments of Tsk^−/+ mice increased K⁶³ ubiquitin on IRF5 in Tsk^−/+ hearts with the levels on IRF5 from treated Tsk^−/+ mice being the highest when compared with levels in C57BL/6J and Tsk^−/+ myocardium.