Additional file 6: of Receptor tyrosine kinase profiling of ischemic heart identifies ROR1 as a potential therapeutic target

Analysis of ROR1 phosphorylation in HL-1 cardiomyocytes after hypoxia and reoxygenation. A) Western analysis of tyrosine phosphorylation after ROR1 immunoprecipitation. Cells were first allowed to adhere for 24 h after plating in normoxic conditions. This was followed by culturing the cells in a hypoxic work station at 1% O2 (hypoxia) and subsequently again in the regular cell incubator in normoxia (reoxygenation) for the indicated periods of time. As different time points were distributed over two days after plating, control samples cultured in normoxia for 24 or 48 h were also analyzed. Time point of one hour of hypoxia (lane 1) is comparable to the 24 h control (lane 2) and time point of one hour of hypoxia and 24 h of reoxygenation (lane 3) is comparable to the 48 h control (lane 4). B) Quantitation of ROR1 phosphorylation relative to total protein. (PDF 179 kb