Additional file 5: of Adipose-derived stem cell-released osteoprotegerin protects cardiomyocytes from reactive oxygen species-induced cell death

Figure S4. Showing that OPG and TRAIL-R3 contribute to the survival of ASCs exposed to ROS. (A) Expression of TRAIL-R3 mRNA 24 hours after transfection of siRNA specific to TRAIL-R3. 18 s-rRNA used as internal control. (B) Effect of OPG and/or TRAIL-R3 downregulation on the viability of ASCs exposed ROS. Cells transfected with OPG siRNA and/or TRAIL-R3 siRNA for 24 hours and then exposed to H2O2 (100 and 200 ÎźM) for an additional 24 hours. *p < 0.05 compared to untreated control. (PDF 87 kb