Immunoblotting shows PEG-FUD significantly decreased fibronectin without degradation in ECM fractions of UUO kidneys.
- Bianca R. Tomasini-Johansson (5892587)
- Pawel W. Zbyszynski (5892590)
- Inger Toraason (5892593)
- Donna M. Peters (5892596)
- Glen S. Kwon (527636)
Publication date
October 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
DOC-insoluble fractions from UUO kidneys (10 μg/lane) from mice treated with saline, PEG, FUD or PEG-FUD were immunoblotted using RamFN IgG at 2 ng/ml and anti-histone3 at 1:10000, followed by anti-rabbit-HRP IgG at 1:10000. Quantitation of fibronectin and histone 3 band intensities was carried out using Image J. The means of fibronectin (FN) bands normalized to H3 from 3 or 5 mice (as indicated) +/- SD are presented. Mouse ID numbers are depicted above corresponding lane. Significance is denoted as * p < 0.05; ** p < 0.01, as per Student t-Test analysis.</p
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