TUNEL-positive apoptotic cells in lung tissue.

(A) Representative confocal images of lung tissues stained with TUNEL (green) with DAPI (blue) (left panel, original magnification; ×200, scale bar; 100μm). The number of TUNEL-positive cells per high power field of each group (right panel). (n = 8, 8, 6, 8, 6 and 8 in WT-NC, WT-HC, WT-HM, FPR1-/- -NC, FPR1-/- -HC and FPR1-/- -HM, respectively) (B) Representative western blots of caspase 9 and glyceraldehyde-3-phos phosphatedehydrogenase (GAPDH; loading control) and densitometric analysis of caspase 9 levels normalized to GAPDH (n = 6, 8, 7, 6, 7 and 6 in WT-NC, WT-HC, WT-HM, FPR1-/- -NC, FPR1-/- -HC and FPR1-/- -HM, respectively) WT-NC, wild type mice of normoxia control; WT-HC, wild type mice of hyperoxia control; WT-HM, wild type mice of hyperoxia with MSCs; FPR1-/- -NC, FPR1 knockout mice of normoxia control; FPR1-/- -HC, FPR1 knockout mice of hyperoxia control; FPR1-/- -HM, FPR1 knockout mice of hyperoxia with MSCs. Data are given as mean ± SEM. * P -/- -NC.