Asn2 cytoophidium is dependent on Asn1.
- Shanshan Zhang (50616)
- Kang Ding (2008447)
- Qing-Ji Shen (5900225)
- Suwen Zhao (1373610)
- Ji-Long Liu (171843)
Publication date
November 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) Confocal images of cytoophidium in A1G, A2G, A1G A2KO, A2G A1KO cells. Scale bar, 2 μm. (B) Quantification of cytoophidium abundance in A1G, A2G, A1G A2KO, A2G A1KO cells showing that Asn1 is crucial for Asn2 cytoophidium formation. ***P<0.0001. n.s. = not significant. Error bars show SEM. (C) Western blot analysis of ASNS protein level and quantification of the relative ASNS abundance in A1G, A2G, A1G A2KO, A2G A1KO cells. n.s. = not significant. Error bars show SEM. (D) Spot assay of A1G, A2G, A1G A2KO and A2G A1KO cells. Eight microliters of four 10-fold serial dilution of each yeast culture was spotted.</p
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