Additional file 1: of Potential therapeutic antibodies targeting specific adiponectin isoforms in rheumatoid arthritis

Screening of hybridoma clones that produced monoclonal antibodies (MAb) against a monomeric recombinant human adiponectin. (A) Supernatant (10 ml) from each clone culture was used to detect human recombinant adiponectin in each lane. Clones in lane 1, 3, and 4 were positive. (B) Positive clones were named and cultured. Culture supernatants were collected and purified by protein G-Sepharose column chromatography as described in Materials and Methods. The purity was determined by Coomassie blue staining after PAGE. (C) The purified protein bands were confirmed as IgGs by Western blot using anti-mouse IgG-HRP.(JPG 34 kb