Additional file 3: of Identification of novel biomarker as citrullinated inter-alpha-trypsin inhibitor heavy chain 4, specifically increased in sera with experimental and rheumatoid arthritis

Figure S3. (a) Serum samples obtained at day 14 from pGIA mice. Western blot analysis was performed without modification of citrulline residues, but no bands of citrullinated proteins were detected (left). Western blot analysis performed with modification of citrulline residues allowed detection of the bands (right). G1–G3 Samples of different mice. (b) Serum of mice immunized with pGPI or CFA control were subjected to the color development reagent assay to measure citrulline content (n = 6). Each symbol represents a single mouse. The horizontal and vertical bars represent the mean and SEM values for the group, respectively. (TIFF 149 kb