A) The number of lipid droplets per 100 μm2 of cell area was counted in stably-transfected MDCK cells. Each sample included at least 10 fields of view from two separate experiments and is presented as the median value in a box and whisker plot. Cell area was determined using ImageJ. B) Transiently-transfected MDCK cells were compared with untransfected neighboring cells at 12 hours after transfection. * p p p t test in comparisons of cells expressing active CPO and control.</p
<p>After cultivating mammary epithelial cells with 100 μM FFA (palmitic or oleic acid) or with FFA-f...
<p>(A) SW13 cells were pretreated with U18666A (30 µM) for 1 h or left untreated (mock). Then, the c...
<p>(A) BODIPY 493/503 stained LDs were counted in stacks of confocal laser scanning microscopy (CLSM...
Lipid droplets and CPO expressed in stably-transfected MDCK cells were visualized by immunofluoresce...
(A) MDCK cells, stably expressing CPO, were incubated for 1 hour with serum-free medium containing 5...
MDCK cells stably expressing CPO were fixed and immunostained with an antibody to CPO (red in A, B a...
<p><b>A.</b> Reduced serum (10% to 2% FCS) was applied to Huh-7 and HeLa cells for 48 hrs. Bodipy 50...
<p>(A) Representative photomicrographs of colocalization of lipid droplets (LDs) with LC3-II in THP-...
AbstractVariation in transfection efficiency observed in different cell-types is poorly understood. ...
<p><b>a</b> PCMDS proliferation in direct contact (co) and in transwell (trw) conditions with FLFCs ...
<p>(A, B) WT-Jc1 or mutant-transfected cells were fixed after 48 h and subjected to immunofluorescen...
<p>A, Panel a shows the percent of Ad-LIP MDA-MB-468 cells that are double positive for GFP and Cell...
(A) MDCK cells stably expressing CPO were treated for the indicated times with cholesterol or (2-Hyd...
Figure S1. Related to Figure 1 (A) Triglycerides measured by enzymatic methods or by flow cytometry ...
<p>Cultured skm cells were transduced with Ad-GFP or Ad-PGC-1α and incubated with 0.5 mM oleate for ...
<p>After cultivating mammary epithelial cells with 100 μM FFA (palmitic or oleic acid) or with FFA-f...
<p>(A) SW13 cells were pretreated with U18666A (30 µM) for 1 h or left untreated (mock). Then, the c...
<p>(A) BODIPY 493/503 stained LDs were counted in stacks of confocal laser scanning microscopy (CLSM...
Lipid droplets and CPO expressed in stably-transfected MDCK cells were visualized by immunofluoresce...
(A) MDCK cells, stably expressing CPO, were incubated for 1 hour with serum-free medium containing 5...
MDCK cells stably expressing CPO were fixed and immunostained with an antibody to CPO (red in A, B a...
<p><b>A.</b> Reduced serum (10% to 2% FCS) was applied to Huh-7 and HeLa cells for 48 hrs. Bodipy 50...
<p>(A) Representative photomicrographs of colocalization of lipid droplets (LDs) with LC3-II in THP-...
AbstractVariation in transfection efficiency observed in different cell-types is poorly understood. ...
<p><b>a</b> PCMDS proliferation in direct contact (co) and in transwell (trw) conditions with FLFCs ...
<p>(A, B) WT-Jc1 or mutant-transfected cells were fixed after 48 h and subjected to immunofluorescen...
<p>A, Panel a shows the percent of Ad-LIP MDA-MB-468 cells that are double positive for GFP and Cell...
(A) MDCK cells stably expressing CPO were treated for the indicated times with cholesterol or (2-Hyd...
Figure S1. Related to Figure 1 (A) Triglycerides measured by enzymatic methods or by flow cytometry ...
<p>Cultured skm cells were transduced with Ad-GFP or Ad-PGC-1α and incubated with 0.5 mM oleate for ...
<p>After cultivating mammary epithelial cells with 100 μM FFA (palmitic or oleic acid) or with FFA-f...
<p>(A) SW13 cells were pretreated with U18666A (30 µM) for 1 h or left untreated (mock). Then, the c...
<p>(A) BODIPY 493/503 stained LDs were counted in stacks of confocal laser scanning microscopy (CLSM...