Phenotypic effects of mutation of <i>HDG3</i> in Col.

(A) In situ hybridization of HDG3 (purple) in seeds from the indicated crosses. Female parent is written first. Scale bars, 50 μm. (B) Endosperm cellularization is slightly delayed in hdg3 compared to wild-type at 5 DAP. For each seed the embryo stage was determined and then the stage of endosperm cellularization was defined as normal, early, or delayed given that embryo stage. (C) Seed weight of wild-type and hdg3 seeds. Individual data points and mean +/- SD shown. P-value from unpaired two-tailed t-test. (D) Seed area is significantly reduced in hdg3 seed (n = 275) compared to wild-type siblings (n = 376) (p = 8.51e-11 by Welch’s two tailed t-test). Seeds were quantified with ImageJ.