Detections of tissue degeneration.

<p><b>(A–D).</b> Evan’s Blue staining of control seeds. (A-C) Transverse sections at 15 DAP, 20 DAP, and 26 DAP, respectively. (D) The longitudinal section at 26 DAP. The blue tissues indicate dead endosperm cells. <b>(E–H).</b> Evan’s Blue staining of m-seeds. (E–G) Transverse sections at 15 DAP, 20 DAP, and 26 DAP respectively. H Longitudinal sections at 26 DAP. Bar = 500 µm in (A) to (H). EM, embryo. <b>(I).</b> Quantitative real-time PCR analyses of PCD-related genes in 7 DAP seeds of control and transgenic plants. <i>ACTIN1</i> was used as an internal control. Error bar indicated the SD (n = 3). ** indicates an extremely significant difference (P<0.01) between control and transgenic lines.