Western blot (WB), immunofluorescence (IF), and immunohistology (IH) of cultured cells using antiserum against C-terminal portion of HISV-1 (anti-HISV NP) or UHV-1 (anti-UHV NP) NP.

A) The panels on right show WBs comparing I/1Ki cells infected with UHV-2 or HISV-1 alone to I/1Ki cells co-infected with UHV-2 and HISV-1. The molecular weight marker (Precision Plus Protein All Blue Prestained Protein Standards, Bio-Rad) is shown in red, and the staining with anti-HISV NP (left) and anti-UHV NP (right) is shown in green. The figure was obtained using Odyssey infrared imaging system (LI-COR). The panels on left show IF staining of I/1Ki cells infected with UHV-2 alone or HISV-1 alone using anti-HISV NP (top panels) and anti-UHV NP (bottom panels). The cells were stained at two days post infection (dpi). The red channel shows NP staining and the blue channel nuclei (DAPI staining). The figures were captured at 400x magnification. B) I/1Ki cells infected with HISV-1 or UHV-2 IF stained at 2 dpi. The top panels show HISV-1 infected cells stained with anti-HISV NP and the bottom panels show UHV-2 infected cells stained with anti-UHV NP. The red channel shows NP staining and the blue channel nuclei (Hoechst staining). The panels on left side were captured at 100x and the panels on right side at 400x magnification. The yellow square indicates the individual cells shown at higher magnification on the rightmost panels. C) IH of non-infected and HISV-1 infected I/1Ki cells. The HISV-1 infected cells were collected and fixed at 6 dpi, the figures were captured at 400x magnification.