Generation of tamoxifen-inducible Cre transgenic mice.

A: Schematic of the RPE-specific Tyr-GFP construct used for generation of Tyr-GFP mice. B: Regional examples (I-IV) of Tyr-GFP expression showing comprehensive expression of GFP under control of the Tyr promoter. Images taken from the flatmount shown in S1 Fig. C: Schematic of the induction of Cre activity in double transgenic mice. Mice harboring the RPE-Tyrosinase-CreErT2 cassette and the Ai14 cassette are treated with tamoxifen to induce Cre activity. Following recombination, the floxed exons (yellow triangles) are excised. In the case of the Ai14 cassette, recombination removes a stop sequence allowing for expression of tdTomato. Scale bar: (B) 50 μm.