Neddylation in T cells contributes to resolution of blood-stage <i>P</i>. <i>yoelii</i> 17XNL infection.

(A) Immunoblotting of total cell extracts from thymocytes, splenic T (CD3+) cells, or B (B220+) cells of Uba3fl/flLck-Cre+ (Uba3ΔT) and Uba3fl/fl mice. T cell-specific Uba3 deficiency was validated by probing with anti-Uba3 antibody. Numbers indicate densitometry of the bands relative to that of Uba3fl/fl mice. (B) Splenic T cells purified from Uba3fl/fl and Uba3ΔT mice were left either untreated or stimulated with CD3/CD28 Dynabeads for 48h, NEDD8-conjugated cullins was detected by probing with anti-NEDD8 antibody. Numbers indicate densitometry of the bands relative to that of unstimulated Uba3fl/fl cells (0 h). (C-D) Groups of Uba3fl/fl and Uba3ΔT mice were infected i.p. with 3×104 parasitized erythrocytes of P. yoelii 17XNL (n = 8 per group), (C) survival curves were analyzed by log-rank test, (D) parasitemia values are presented as mean±SEM and analyzed by Mann-Whitney U test. (E-F) Course of P. yoelii 17XNL infection in Nedd8fl/fl and Nedd8ΔT mice (n = 9 per group), overall survival and parasitemia were analyzed as described above. Data shown are from one of three independent experiments with similar results. * ppp<0.001.