Neddylation in CD4⁺ T cells plays a crucial role in anti-<i>P</i>. <i>yoelii</i> 17XNL immunity.

(A) Validation of Uba3 expression in splenic CD4+ T cells from tamoxifen-treated Uba3fl/fl and Uba3fl/flCd4-CreERT2 (Uba3-iKO) mice. Numbers indicate densitometry of the bands relative to that of Uba3fl/fl mice. (B) Numbers of total CD4+ T cells and activated CD44hiCD4+ T cells in spleens of Uba3fl/fl and Uba3-iKO mice prior to P. yoelii 17XNL infection (n = 4 per group). (C) Serum levels of IFN-γ in Uba3fl/fl and Uba3-iKO mice at days 0, 5, 9 p.i. (n = 5–6 per group). (D) Serum levels of anti-P. yoelii 17XNL IgG in Uba3fl/fl and Uba3-iKO mice during the course of infection (n = 5–6 per group). (E-F) Representative dot plots and bar graphs showing the proportions and total numbers of (E) GC B cells, and (F) plasma cells in spleens of Uba3fl/fl and Uba3-iKO mice at day 16 p.i. (n = 4 per group). Data are representative of three replicate experiments and expressed as mean±SEM. *pppt test.