Innate immunity of DDX3X-deficient cells and of <i>Ddx3x</i>^{fl/y <i>Vav-iCre</i>} mice to VSV and <i>Listeria monocytogenes infection</i>.

A, B WT MEFs, or MEFs derived from Ddx3xfl/y CreERT2 mice and treated with 4-OHT to delete Ddx3x were infected with VSV at an MOI of 0,1. After the indicated periods, RNA was isolated and pan-Ifna (A) or Ifnb (B) mRNA expression was determined by qPCR. Lines represent the mean +/- standard deviation (SD). Statistical significance was calculated using the unpaired, two-tailed Student's t-test. *PC Supernatants from cells infected with VSV at an MOI of 0.1 were collected and viral loads at the indicated times were measured by plaque-forming unit (pfu) assay. Lines represent the mean +/- standard deviation (SD). Statistical significance was calculated using the unpaired, two-tailed Student's t-test. *PD Ddx3xfl/y and Ddx3xfl/y Vav-iCre mice (n = 7) were infected intravenously with 106 pfu of VSV. Survival was monitored for the indicated period of time. Statistical significance was calculated using the Log-rank (Mantel-Cox) test and Gehan-Breslow-Wilcoxon test. Differences between mouse genotypes did not reach statistical significance. E Ddx3xfl/y and Ddx3xfl/y Vav-iCre mice (n = 10) were injected intraperitoneally with 1,6x105 CFU L. monocytogenes (strain EGD). Survival was monitored daily for the indicated period. Statistical significance was calculated using the Log-rank (Mantel-Cox) test (P-value: F Ddx3xfl/y mice (n = 14) and Ddx3xfl/y Vav-iCre mice (n = 13) were injected intraperitoneally with 8x104 CFU L. monocytogenes (strain EGD). After 72 h, mice were sacrificed and the bacterial load was determined in spleen and liver. Pooled data from three independent experiments is shown. Lines represent the mean +/- standard error of the mean (SEM). P-values were calculated using the unpaired, two-tailed Student's t-test. *P<0.05, **P<0.01, ***P<0.005.