<i>Ddx3x</i>^{fl/y <i>Vav-iCre</i>} mice show alterations in spleen and peritoneum upon <i>Listeria monocytogenes</i> infection.

ADdx3xfl/y and Ddx3xfl/y Vav-iCre mice were infected intraperitoneally with 1x105 CFU Listeria monocytogenes (strain EGD) for 24 h, spleens were isolated, homogenized and stained with the indicated surface markers, followed by flow cytometry. Total cell numbers per organ are shown (n = 16 for Ddx3xfl/y and 20 for Ddx3xfl/y Vav-iCre mice for total, B220+ and CD3+ cells, analyzed in 5 independent experiments; n = 4/8 for CD4+, CD8+ and CD1d-tetramer+ cells, all pre-gated on CD3+, analyzed in 2 independent experiments; n = 4/8 for NK1.1+ cells (pre-gated on CD3-), analyzed in two independent experiments; n = 12/12 for CD11c+, CD11b+F4/80+ cells, analyzed in 3 independent experiments; n = 12/12 for Ly6G+Ly6C-/lo and Ly6GloLy6Chi cell populations, both pre-gated on CD11b+and analyzed in 3 independent experiments). B Ddx3xfl/y mice and Ddx3xfl/y Vav-iCre mice were infected intraperitoneally with 1x105 CFU L. monocytogenes (strain EGD) for 24h or treated with PBS as a control. Peritoneal exudate cells were collected via peritoneal lavage and stained with the indicated surface markers. Total cell numbers of B220+ B cells, CD3+ T cells, Ly6G+Ly6Clo neutrophils and Ly6G-/lo Ly6Chi monocytes (both pre-gated on CD11b+) and CD11b+ F4/80+ macrophages are shown. n = 7 (Ddx3xfl/y mice) and 5 (Ddx3xfl/y Vav-iCre mice) for PBS controls and n = 12/11 for infected animals. P-values were calculated using the unpaired, two-tailed Student's t-test. Mean and standard error of the mean (SEM) are indicated in the graphs. *P<0.05, **P<0.01, ***P<0.005.