WDR68 is required for normal levels of DYRK1B.

A) RT-qPCR analysis of Dyrk1b mRNA expression in C2C12 sublines. Column 1 is NT1 cells in GM. Column 2 is NT1 cells in DM. Column 3 is Δwdr68-9 (Δ9) cells in DM. Column 4 is Δdyrk1a-12 (Δ12) cells in DM. The bottom graph shows the individual ΔCt values (circles) of replicates and the average ΔCt (horizontal bar) -/+ standard deviations. The top graph shows the 2ΔΔCt values (fold change relative to NT1 GM) with corresponding low and high ranges. B) Western blot analysis of C2C12 NT1 and Δwdr68-9 cells in DM. DYRK1B panel: Lane 1, DYRK1B was readily detected in NT1 cells. Lane 2, DYRK1B expression was reduced in Δwdr68-9 cells. β-tubulin panel: β-tubulin controls indicated similar loading in each lane. B’) Quantitative analysis confirmed significantly reduced DYRK1B expression in the Δwdr68 subline. Three independent biological replicates were analyzed.