B-factors plots.

A plot of theoretical B-factors as calculated by Seq-GNM (blue), the original GNM obtained from structure (orange), and observed experimental B-factors (black) for acyl-CoA dehydrogenase (PDB id: 1JQI) along with predicted contact maps by Seq-GNM (using a threshold score, shown as blue) and the contact map of the structure (using 10Å cut-off distance) (a) The Seq-GNM with values obtained from RaptorX produces a correlation of 0.56 with experiment, and 0.77 with the GNM obtained from structure. Moreover, the contact maps reveal that the predicted contacts between the Seq-GNM and structural GNM approaches are remarkably similar. (b) The Seq-GNM that uses values obtained from EVcouplings produces a correlation of 0.60 with experiment, and 0.68 with the GNM obtained from structure. The B-factor obtained by applying GNM to the experimental structure yields a correlation of 0.57. The contact map captures the dominant contacts with noise coming from poorly predicted EVcouplings scores.