Effect of ARH1 genotype and gender on ADP-ribosylarginine content.

ADP-ribosylarginine content of proteins from whole intestine (upper) or epithelial cells (lower) of loops were incubated with PBS or 0.5 μg of CT for 6 h. Data are means ± SEM of values (n = 6) from two loops of two mice in each of three experiments. Effects of genotype (p < 0.001), gender (p < 0.001) or treatment (p < 0.001) are significant for ADP-ribosylarginine content of proteins (2-way ANOVA, Tukey’s multiple comparison test). In whole intestine, pairwise comparisons were significantly different in KO male or female CT vs. KO male or female PBS (p < 0.001), KO female CT vs. KO male CT (p = 0.001) or KO female PBS vs KO male PBS (p = 0.0393), but differences were not significant (ns) for WT female CT vs. WT male CT (p = 0.9625) or WT female PBS vs. WT male PBS mice (p = 0.8972). In epithelial cells, differences were significant in KO male or female CT vs. KO male or female PBS (p < 0.001), KO female CT vs. KO male CT (p < 0.001), but not significant (ns) in WT female CT vs. WT male CT (p = 0.5815) or WT female PBS vs. WT male PBS mice (p = 0.9996).