Elevated urinary protein concentration in <i>Mras</i>^<i>-/-</i> males.

<p>(A) The protein concentration of randomly collected urine samples was elevated in <i>Mras</i>^<i>-/-</i> males; t-test: <i>p</i> = 0.0115. (B) Water consumption by young (8 week-old) and older (26 week-old) males. Two-way ANOVA: There was a significant effect of genotype, F_1,20 = 18.23, <i>p</i> = 0.0004, but a significant interaction between genotype and age was also detected (<i>p</i> = 0.0407). The difference in water consumption was significant at 26 weeks (<i>p</i> = 0.0011). Eight week-old mice: WT and <i>Mras</i>^<i>-/-</i>, n = 6; 26 week-old mice: WT, n = 5; <i>Mras</i>^<i>-/-</i>, n = 7. Numbers represent cages of group-housed mice but water consumption was calculated on average per mouse. Error bars represent SD. (C) Creatinine levels were similar in WT and <i>Mras</i>^<i>-/-</i> males; t-test: not significant. (D) Normalized to creatinine, protein content in <i>Mras</i>^<i>-/-</i> urine was not significantly different from that of WT urine; (A, C, D) WT, n = 9; <i>Mras</i>^<i>-/-</i>, n = 13; error bars represent SEM.