Mucosal rectal ion flux in response to serosal and mucosal application of inhibitors to everted tissues.

Mucosal Mg2+ (A), Ca2+ (B) and H+ (C) flux in response to serosal and mucosal application of Cortland saline containing 0.1mM ouabain, 0.1mM Co3Hex or both inhibitors to the everted esophagus from unfed C. auratus. Values are relative to the control treatment (symmetrical application of Cortland saline). Salines used on both surfaces were buffered with 10mM HEPES (pH = 7.4). All measurements were taken at the mucosal surface. Data are mean flux ± S.E.M. N = 7. A repeated measures one-way ANOVA (inhibitor as factor) and post-hoc Holm-Sidak test was used to compare individual treatments to the control (* indicates significant difference (p<0.05) from control).